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anti cd68 antibody  (Elabscience Biotechnology)
94
Elabscience Biotechnology anti cd68 antibody
MMP12 silencing inhibited M2 macrophage polarization. THP-1 cells were differentiated into M0 macrophages (THP-1 M0) by treatment with 100 ng/mL PMA for 24 h. (A) Flow cytometry was used to quantify the number of <t>CD68-positive</t> cells. Subsequently, KYSE150 cells were co-cultured with the THP-1-derived macrophages using a Transwell system. (B) The mRNA levels of IL-10, Arg-1, and TGF-β were detected by qRT-PCR. (C) Flow cytometry was used to quantify the number of CD206-positive macrophages. (D) Cell migration analysis by transwell migration assay. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.
Anti Cd68 Antibody, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd68 antibody - by Bioz Stars, 2026-06
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resource source identifier cd68 d4b9c xp rabbit mab cell signaling technology rrid ab 2799882  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc resource source identifier cd68 d4b9c xp rabbit mab cell signaling technology rrid ab 2799882
MMP12 silencing inhibited M2 macrophage polarization. THP-1 cells were differentiated into M0 macrophages (THP-1 M0) by treatment with 100 ng/mL PMA for 24 h. (A) Flow cytometry was used to quantify the number of <t>CD68-positive</t> cells. Subsequently, KYSE150 cells were co-cultured with the THP-1-derived macrophages using a Transwell system. (B) The mRNA levels of IL-10, Arg-1, and TGF-β were detected by qRT-PCR. (C) Flow cytometry was used to quantify the number of CD206-positive macrophages. (D) Cell migration analysis by transwell migration assay. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.
Resource Source Identifier Cd68 D4b9c Xp Rabbit Mab Cell Signaling Technology Rrid Ab 2799882, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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resource source identifier cd68 d4b9c xp rabbit mab cell signaling technology rrid ab 2799882 - by Bioz Stars, 2026-06
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cd68  (Elabscience Biotechnology)
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Elabscience Biotechnology cd68
The expression levels of both STAT5A and COL4A2 were upregulated in M2 macrophages within the ICP cell model. ( A ) Flow cytometric analysis of THP-1 cell typing indicators CD206 and <t>CD68,</t> n = 3. ( B ) Proliferation of M2 macrophages following TCA treatment was evaluated using the CCK-8 assay. ( C ) Western blot (WB) analysis of STAT5A expression in the ICP cell model, n = 3. ( D ) WB analysis of COL4A2 expression in the ICP cell model, n = 3. ( E ) WB detection of TGF-β expression in the ICP cell model, n = 3. (F) WB assessment of SMAD expression in the ICP cell model, n = 3
Cd68, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd86  (Elabscience Biotechnology)
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Elabscience Biotechnology cd86
The expression levels of both STAT5A and COL4A2 were upregulated in M2 macrophages within the ICP cell model. ( A ) Flow cytometric analysis of THP-1 cell typing indicators CD206 and <t>CD68,</t> n = 3. ( B ) Proliferation of M2 macrophages following TCA treatment was evaluated using the CCK-8 assay. ( C ) Western blot (WB) analysis of STAT5A expression in the ICP cell model, n = 3. ( D ) WB analysis of COL4A2 expression in the ICP cell model, n = 3. ( E ) WB detection of TGF-β expression in the ICP cell model, n = 3. (F) WB assessment of SMAD expression in the ICP cell model, n = 3
Cd86, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd68 antibodies  (Elabscience Biotechnology)
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Elabscience Biotechnology cd68 antibodies
The expression levels of both STAT5A and COL4A2 were upregulated in M2 macrophages within the ICP cell model. ( A ) Flow cytometric analysis of THP-1 cell typing indicators CD206 and <t>CD68,</t> n = 3. ( B ) Proliferation of M2 macrophages following TCA treatment was evaluated using the CCK-8 assay. ( C ) Western blot (WB) analysis of STAT5A expression in the ICP cell model, n = 3. ( D ) WB analysis of COL4A2 expression in the ICP cell model, n = 3. ( E ) WB detection of TGF-β expression in the ICP cell model, n = 3. (F) WB assessment of SMAD expression in the ICP cell model, n = 3
Cd68 Antibodies, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody ab 2892065  (Bio-Rad)
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Bio-Rad antibody ab 2892065
The expression levels of both STAT5A and COL4A2 were upregulated in M2 macrophages within the ICP cell model. ( A ) Flow cytometric analysis of THP-1 cell typing indicators CD206 and <t>CD68,</t> n = 3. ( B ) Proliferation of M2 macrophages following TCA treatment was evaluated using the CCK-8 assay. ( C ) Western blot (WB) analysis of STAT5A expression in the ICP cell model, n = 3. ( D ) WB analysis of COL4A2 expression in the ICP cell model, n = 3. ( E ) WB detection of TGF-β expression in the ICP cell model, n = 3. (F) WB assessment of SMAD expression in the ICP cell model, n = 3
Antibody Ab 2892065, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MMP12 silencing inhibited M2 macrophage polarization. THP-1 cells were differentiated into M0 macrophages (THP-1 M0) by treatment with 100 ng/mL PMA for 24 h. (A) Flow cytometry was used to quantify the number of CD68-positive cells. Subsequently, KYSE150 cells were co-cultured with the THP-1-derived macrophages using a Transwell system. (B) The mRNA levels of IL-10, Arg-1, and TGF-β were detected by qRT-PCR. (C) Flow cytometry was used to quantify the number of CD206-positive macrophages. (D) Cell migration analysis by transwell migration assay. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

Journal: Regenerative Therapy

Article Title: WTAP stabilizes MMP12 expression to promote the malignant phenotypes of esophageal cancer cells

doi: 10.1016/j.reth.2026.101101

Figure Lengend Snippet: MMP12 silencing inhibited M2 macrophage polarization. THP-1 cells were differentiated into M0 macrophages (THP-1 M0) by treatment with 100 ng/mL PMA for 24 h. (A) Flow cytometry was used to quantify the number of CD68-positive cells. Subsequently, KYSE150 cells were co-cultured with the THP-1-derived macrophages using a Transwell system. (B) The mRNA levels of IL-10, Arg-1, and TGF-β were detected by qRT-PCR. (C) Flow cytometry was used to quantify the number of CD206-positive macrophages. (D) Cell migration analysis by transwell migration assay. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

Article Snippet: For immunophenotyping, single-cell suspensions of both THP-1 and THP-1-M0 cells were labeled with an anti-CD68 antibody (E-AB-F1299L, Elabscience, Wuhan, China).

Techniques: Flow Cytometry, Cell Culture, Derivative Assay, Quantitative RT-PCR, Migration, Transwell Migration Assay

The expression levels of both STAT5A and COL4A2 were upregulated in M2 macrophages within the ICP cell model. ( A ) Flow cytometric analysis of THP-1 cell typing indicators CD206 and CD68, n = 3. ( B ) Proliferation of M2 macrophages following TCA treatment was evaluated using the CCK-8 assay. ( C ) Western blot (WB) analysis of STAT5A expression in the ICP cell model, n = 3. ( D ) WB analysis of COL4A2 expression in the ICP cell model, n = 3. ( E ) WB detection of TGF-β expression in the ICP cell model, n = 3. (F) WB assessment of SMAD expression in the ICP cell model, n = 3

Journal: Journal of Translational Medicine

Article Title: Metamorphosing macrophages promote placental fibrosis by upregulating the transcription factor STAT5A

doi: 10.1186/s12967-026-07727-9

Figure Lengend Snippet: The expression levels of both STAT5A and COL4A2 were upregulated in M2 macrophages within the ICP cell model. ( A ) Flow cytometric analysis of THP-1 cell typing indicators CD206 and CD68, n = 3. ( B ) Proliferation of M2 macrophages following TCA treatment was evaluated using the CCK-8 assay. ( C ) Western blot (WB) analysis of STAT5A expression in the ICP cell model, n = 3. ( D ) WB analysis of COL4A2 expression in the ICP cell model, n = 3. ( E ) WB detection of TGF-β expression in the ICP cell model, n = 3. (F) WB assessment of SMAD expression in the ICP cell model, n = 3

Article Snippet: Subsequently, the cells were incubated with flow cytometry antibodies CD206 (Elabscience, E-AB-F1161E) and CD68 (Elabscience, E-AB-F1299L) to be labeled for identification through a BD Canto system (USA).

Techniques: Expressing, CCK-8 Assay, Western Blot